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What is the last step in the process of genetic engineering described?

Identify gene

Cut gene using restriction enzyme

Insert plasmid into target cell

In the process of genetic engineering, inserting a plasmid into a target cell is indeed one of the final steps. After identifying the gene of interest and cutting it using restriction enzymes, the next logical step is to insert the plasmid – which contains the desired gene – into the target cell. This step is crucial for ensuring that the new genetic material can be expressed by the host organism, allowing it to potentially exhibit new traits or characteristics.

Once the plasmid is inside the target cell, it can replicate during cell division and be expressed, but the insertion itself represents a pivotal moment in the genetic engineering process. It serves as a bridge between manipulating genetic material and observing the results of that manipulation in an organism. This step allows researchers to move forward in culturing the cells to produce clones, so the goal of genetic engineering can be realized.

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Culture cell by cloning

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